Yields of 2-Deoxy-D-gluconic, D-Gluconic and Other Sugar Acids in Gamma-Irradiated Aqueous Solutions of D-Glucose

The yields of 2-deoxy-D-gluconic, D-gluconic and other sugar acids from 6 0 Co-gamma irradiated (dose rate = 4 Krads/min) D-glucose solutions are reported. The acids produced upon radiolyses were separated from glucose and neutral products by anion exchange, assayed by gas chromatography of the trimethylsilyl derivatives, and definitive identification made by mass spectrometry. In He degassed, irradiated 0.055 M glucose G(2-deoxy-Dgluconic acid) = 0.62 and G(D-gluconic acid) = 0.20. The approximate G values for the other identified acids are: glyceric acid 0.03, 2-deoxy-tetronic acid 0.04, tetronic acid 0.03, 4-deoxypentonic acid 0.02, deoxyketogluconic acid 0.17. In N20 saturated glucose solutions D-gluconic acid yields increased by a factor of ~ 1.9 while that of 2-deoxy-D-gluconic acid increased by a factor of only ~ 1.1.